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2 edition of Serological identification of common and strain-specific antigens in Agrobacterium found in the catalog.

Serological identification of common and strain-specific antigens in Agrobacterium

HaceМЂne Bouzar

Serological identification of common and strain-specific antigens in Agrobacterium

by HaceМЂne Bouzar

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  • 7 Currently reading

Published .
Written in English

    Subjects:
  • Agrobacterium.,
  • Rhizobium.,
  • Immunospecificity.

  • Edition Notes

    Statementby Hacene Bouzar.
    The Physical Object
    Pagination88 leaves, bound :
    Number of Pages88
    ID Numbers
    Open LibraryOL15528111M

    CFA/II is composed of coli surface (CS) antigens CS1, CS2 and CS3, whereas CFA/IV is composed of CS4, CS5, and CS6 (10, 11, 13). In addition, several other CFAs have been identified, namely, CS12, CS14, CS17, and CS Serological Identification of Patient's Antibodies The serology laboratory tests patients’ sera to detect specific antibodies. The results of such tests may provide a “serological diagnosis” of an infectious disease in which antibody was produced in specific response to the microbial antigens .

    We sought to characterize the strain-specific antigens of four strains of Mycoplasma pulmonis (47, 63, Negroni, and ) by crossed immunoelectrophoresis. Although the strains possessed a number of common antigens, type-specific antigens of mobility (bovine albumin was assigned a value of 1) were detected in strains 47 and Strain-specific ELISA was performed on PolySorp microtiter plates (Nunc, Roskilde, Denmark). The wells of the plates were coated overnight with 50 μl of purified gH antigens (antigens AP86 and TO86) or gB antigens (antigens AD55 and TO55) diluted in carbonate buffer and blocked with 3% goat serum in borate buffer (BB) for 2 h at 37° samples diluted in BB were added to the wells.

    Abstract. Cultural and physiological properties, serology, plasmid profiles and infective traits were determined for 23 strains of rhizobia isolated from various Hedysarum species: H. coronarium (common name: sulla) (16), H. carnosum (1), H. alpinum (3), H. mackenzii (2) and H. pallens (1) from Portugal, Spain, Tunisia, Alaska and Israel. Strains isolated from H. alpinum, H. mackenzii and H. Identification methods are not exhaustively reviewed because they should require an entire review by them-selves, but some key common aspects are discussed af-ter the diagnostic and detection methods. Corresponding author: M.M. López Fax: + E-mail: [email protected] _Lopez_S57 Pagina


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Serological identification of common and strain-specific antigens in Agrobacterium by HaceМЂne Bouzar Download PDF EPUB FB2

The serological uniqueness of each strain was confirmed by EcoRI digestion profiles of total DNA. The profiles were different for each of the Agrobacterium strains. The utility of the broadly specific method was demonstrated when antisera to 50 S ribosomal subunits were used successfully to identify putative agrobacteria isolated from a natural : Hacene Bouzar.

SEROLOGICAL IDENTIFICATION OF COMMON AND STRAIN-SPECIFIC ANTIGENS IN AGROBACTERIUM by Hacene Bouzar A THESIS submitted to Oregon State University in partial fulfillment of the requirements for the degree of Doctor of Philosophy Completed Febru Commencement June The strain-specific\ud serological reaction was due to lipopolysaccharide antigens present\ud as contaminants in the ribosomal preparation.

The serological\ud uniqueness of each strain was confirmed by EcoRI digestion profiles\ud of total DNA. The profiles were different for each of the\ud Agrobacterium. Serological techniques and restriction enzyme cleavage patterns of total DNA were used to differentiate strains of Agrobacterium spp.

Forty-five wild-type and plasmid-cured Agrobacterium strains. Abstract. Serological comparison of biotypes 1, 2, and 3 of Agrobacterium was made by double immunodiffusion (DID), indirect immunofluorescence (IIF), and the indirect enzyme-linked immunosorbent assay, (ELISA-I).

Twenty four strains, representing of the three biotype and ten antisera obtained from sonicated whole cells, 0 antigens and glycoprotein extracts were : B. Alarcon, M. Lopez, M. Cambra, M. Gorris. Serological techniques and restriction enzyme cleavage patterns of total DNA were used to differentiate strains of Agrobacterium spp.

Forty-five wild-type and plasmid-cured Agrobacterium strains were tested by immunodiffusion and immunofluorescence against polyclonal antisera to a crude ribosome preparation from Agrobacterium strains K84, U11, B6, A, NT1, and C Whereas the common surface antigen is a likely target for conventional serological reactions used for identification of the species M.

arginini, strain-specific antigen cannot fulfill this role but must participate in other surface reactions. Strain-specific serological techniques for the identification of Rhizobium meliloti in commercial alfalfa inoculants Article in Canadian Journal of Microbiology 29(2) February with.

SDS-polyacrylamide gel electrophoresis profiles of membrane proteins of the Rhizobium and Agrobacterium strains used in this study. of the antigen groups recognized by a. Serological identification of tumour antigens by recombinant expression cloning has proved to be an effective strategy for the identification of cancer-associated genes having a relevance to.

Physiological Plant Patholo() 4, Crown gall induction: serological reactions, isozyme patterns and sensitivity to mitomycin C and to bacteriocin, of pathogenic and non-pathogenic strains of Agrobacterium radiobacter W.

ROBERTs and A. KERR Department of Plant Pathology, Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, South Australia. veterinary parasitology ELSEVIER Veterinary Parasitology 60 () Identification of an immunodominant 40 kDa merozoite antigen common to the Australian T and Dixie vaccine strains of Babesia boris and the development of diagnostic tests specific for these strains J.B.

Molloy a, S.J. Waldron b, W.K. Jorgensen b a Queensland Department of Primary Industries, Animal. Agrobacterium strains Agrobacterium compatibility.

Due to the different degree of spontaneous antibiotics resistance in different Agrobacterium strains, it is critical for choosing right host cells for different vector, and antibiotics. GV(pMP90RK) Most frequently used in Koiwa's lab. A double-antidody sandwich method of enzyme-linked immunosorbent assay (ELISA) previously used for serotyping of peanut Rhizobium isolates was applied for serological identification of fast- and.

The cell envelope antigens of a pathogenic strain (57P) of Agrobacterium tumefaciens were compared with those of a non-pathogenic strain (57sr) by means of quantitative Immunoelectrophoresis. Strain 57P was derived from 57sr by conjugative transfer of the tumour inducing (Ti) plasmid from strain B6Sm, and should differ from 57sr only with respect to characters encoded by.

Bacterial Identification Tests MICROBIOLOGY MODULE Microbiology Notes zBiochemical testing zSerological tests zPhage typing zIdentification disc testing zSemiautomated and Automated identification systems zMolecular techniques (i) Staining of the isolated bacteria Staining of the bacteria forms the foremost and the most important step in the.

~~~~~IDENTIFICATION OF AGROBACTERIUM STRAINS TABLE 1. Plasmids used as probes in this study Plasmid Characteristics Origin Reference pGV pTiC58 Hindlll fragme 31b, 23, 33, 2, and 30b T-DNA 7 pGV pTiC58 Hindlll fragments 14b, 19, 41, 22, and 31b T-DNA 7 pGV pTiC58 HindIll fragme 15, and 14b T-DNA 7.

• Serological characteristics: precipitation test, agglutination, immunofluorescence and ELISA. • Bacteriophage: are too specific and a strain level identification is possible so it is considered as both conventional as well as the modern method of identification and the method is known as Phage plaque technique.

Modern identification methods. Serological comparison of antigens related to local virus and bacterial infections and aspecific stresses in tobacco leaves. Phytopathol., CrossRef | El-Kady, S. and S. Sule, Serological comparison between strains of Agrobacterium radiobacter.

Acta Phytopathologica Academiae Scientiarum Hungaricae, Abstract. Hybridoma cell lines were derived from the fusion of mouse myeloma cells with spleen cells from a mouse that had been immunized withAgrobacterium tumefaciens strain A, a flagella-less mutant of strain C58 containing the Ti plasmid of strain B6.

All of the 20 antibodies produced by the cloned hybridomas reacted with strain C58 and with other strains derived from C. 4). DISCUSSION Recombinant DNA techniques and serological methods were used to show that cell surface specific components of P.

syringae strain R32 are involved in pathogenicity. Purging P. syringae strain R32 cell surface-specific AB, resulted in the serological identification of pathogenicity-related cell surface antigens.In his study of the extra­cellular soluble antigens of 2 strains of R. meliloti, Dudman (36) found that the two strains examined shared all antigens accept several fast-moving ones.

He proposed that since the strains did not cross-agglutinate, that these strain-specific antigens could be used for identification .Abstract. Immunoassays employ a range of methods to detect and quantify antigens or antibodies and to study the composition of antigens.

This chapter describes four useful immunoassays for serological characterization of antigens of Neisseria meningitidis: whole-cell enzyme-linked immunosorbent assay (ELISA); dot blot, colony blot, and immunoblot. Serological characterization of N.